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1983-11-01 1995-01-01 ruvB mutation in a recBCsbcB(C) background. Both conjugational recombination efficiency and MMS damaged DNA repair proficiency returned to wild-type levels in the AuwDruvB9double mutant. Suppres- sion of the effects of the ruvB mutation by a uvrD deletion was dependent on the recG and recN genes I think it depends on the system and conditions you have, but please see if any of these papers may be of any help. Hall, J Mol Evol 1995 40, 86-93, 1995; Tang & Smith, Mutat Res 80, 15-25, 1981 1988-04-01 Mutation of the uvrD gene of Escherichia coli is associated with an increased capacity for genetic recombination. The hyper-recombination effect is abolished by an additional mutation in lexA that limits synthesis of RecA protein and other gene products regulated by LexA repressor, and is not restored when increased synthesis of RecA protein is facilitated by a recAoc mutation. Europe PMC is an archive of life sciences journal literature. Please help EMBL-EBI keep the data flowing to the scientific community!

Uvrd gene mutation

uvrD mutants occurs via the RecF pathway. In uvrD+ cells, constitutive expression of SOS genes is not sufficient to stimulate deletion formation. This suggests that the RecF recombination pathway activated by SOS induction is antagonized by the UvrD protein. Paradoxically, we observed that the overproduction of UvrD from These results were consistent with the idea that the uvrD, uvrE, recL, and pdeB mutations are alleles of the uvrD gene.

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A 2.9-kilobase (kb) Pvu II DNA fragment that contains the uvrD gene of Escherichia coli K-12 has been cloned in both low-copy and multiple-copy plasmid vehicles. The low-copy uvrD plasmid (pVMK49) complements a variety of uvrD, uvrE, and recL mutations. In contrast, the same strains carrying the 2.9-kb fragment in a multiple-copy plasmid (pVMK45) remain sensitive to ultraviolet light (UV To exclude mutations in other genes than the uvrD, we started from a uvrD3 mutant strain, and isolated uvrD-ts mutants by intragenic sup- pression of the uvrD3 mutation. A kinetic study of uvrD gene expression in vivo and the identifica- tion of a suppressor mutation in one of the UV-ts uvrD mutants will be also described.

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The uvrD gene of Escherichia coli that control UV sensitivity and spontaneous mutation frequency has been cloned with phage λ as vector. The increased sensitivity to ultraviolet light (UV) of uvrD3, uvrE502, recL152, and pdeB41 mutants, high mutability of uvrD3 and pdeB41 mutants, and conditional lethality of strain TS41 that carried pdeB41, polA1, and sup126 mutations were all A 2.9-kilobase (kb) Pvu II DNA fragment that contains the uvrD gene of Escherichia coli K-12 has been cloned in both low-copy and multiple-copy plasmid vehicles. The low-copy uvrD plasmid (pVMK49) complements a variety of uvrD, uvrE, and recL mutations. In contrast, the same strains carrying the 2.9-kb fragment in a multiple-copy plasmid (pVMK45) remain sensitive to ultraviolet light (UV Constitutive and SOS-induced levels of uvrD mRNA were determined by S1 mapping, and it was shown that the majority of uvrD transcripts are from P1, while P2 plays only a minor role. A series of increasing deletions from the 5' end of the uvrD gene was used to assay transcription in the promoterless vector pKO-1. Table 2 shows the results for >250 mutations in the D. radiodurans rpoB gene that result in the Rif r phenotype, including 185 spontaneous mutations derived in a wild-type background, 33 NTG-induced mutations, and 19 5AZ-induced mutations, as well as 17 spontaneous mutations occurring in a uvrD background.

Contact Information; Charges; Reviving Strains; Strains Not Found? Other CGSC Information. FAQ on Procedures; Current Working Map; 1998 MMBR Map; 1998 MMBR Gene List ; Map Diagrams (PDFs) Other uvrD mutants retain a limited ability to carry out nucleotide excision repair, and remain proficient in repairing 6-4 photoproducts, which are removed preferentially before cyclobutane pyrimidine dimers or lesions in transcribed genes . The recF143 allele did not alter the phenotypes of dam mutants of Escherichia coli. The uvrD3, uvrE502, and recL152 mutations did alter some of the phenotypes of dam bacteria.
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This suggests that the RecF recombination pathway activated by SOS induction is antagonized by the UvrD protein. Paradoxically, we observed that the overproduction of UvrD from These results were consistent with the idea that the uvrD, uvrE, recL, and pdeB mutations are alleles of the uvrD gene. In addition to the uvrD gene, lambda uvrD+ carried the corA gene that controls transport of Mg++, Mn++, and Co++ through the cell membrane.

To exclude mutations in other genes than the uvrD, we started from a uvrD3 mutant strain, and isolated uvrD-ts mutants by intragenic sup- pression of the uvrD3 mutation.
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Cancer can re 文献「Molecular cloning of the uvrD gene of Escherichia coli that controls ultraviolet sensitivity and spontaneous mutation frequency」の詳細情報です。J-GLOBAL 科学技術総合リンクセンターは研究者、文献、特許などの情報をつなぐことで、異分野の知や意外な発見などを支援する新しいサービスです。 Most genes belong to larger gene families of shared ancestry, detectable by their sequence homology. Novel genes are produced by several methods, commonly through the duplication and mutation of an ancestral gene, or by recombining parts of different genes to form new combinations with new functions. An E coli strain has a loss of function mutation in the gene coding for UvrD from BIOL 2500 at Rensselaer Polytechnic Institute Gene Mutation DNA damages produced by these environmental stresses are thought to induce DNA double-strand breaks, leading to illegitimate recombination. In this paper we show that UV-induced illegitimate recombination is enhanced by mutations of nucleotide excision repair genes, uvrA or uvrB, and partially by uvrC mutation, but not by uvrD mutation.

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1988-07-01 I think it depends on the system and conditions you have, but please see if any of these papers may be of any help. Hall, J Mol Evol 1995 40, 86-93, 1995; Tang & Smith, Mutat Res 80, 15-25, 1981 In this report, we focus on the UvrD homolog in Helicobacter pylori , a genetically diverse organism that lacks many known DNA repair proteins, including those involved in mismatch repair and recombinational repair, and that is noted for high levels of inter- and intragenomic recombination and mutation. Genetisk variation betraktas som en nivå av biologisk mångfald. Det är ett mått på hur genetiskt olika individer är inom en hel art eller en population av en art.